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Check that your experimental setup comforms to a
You have a single factor treatment design (or no treatment design), if you consider your treatments to not be divisible into factors. The treatments are all "just treatments". Plant example: You are applying treatments to plots (experimental units), and all plots are similar in moisture, soil type, slope, fertility, etc.(this makes it a RCBD). You have 5 levels of nitrogen fertilizer, that are all representative of the same single factor (nitrogen). Animal example: You are applying treatments to animals (experimental units), and all animals are similar in weight, age, breed, etc. (this makes it a RCBD). You have 4 protein level diets, that are all representative of the same single factor (protein). In particular, the 4 diets are not created by combining protein source and amount (i.e. 2 factors).
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